Tients with diabetes. Strategies: Individuals at Concord Hospital with suspected CAD gave written informed consent

Tients with diabetes. Strategies: Individuals at Concord Hospital with suspected CAD gave written informed consent and had been administered RIPC (sphygmomanometer around the arm, 3 5 min cycles, n = 31) or sham (n = 29) prior to angiography, with recruitment ongoing. Blood was collected pre- and right away post-RIPC/sham and plateletfree plasma generated. Worldwide coagulation/fibrinolytic potential was measured by overall haemostatic possible assay (Reddel et al. Thromb Res. 2013; 131(five): 457462) and numerous fibrinolytic aspects by ELISA. EV wereUniversity College Dublin, Dublin, Ireland; bQueen Mary University of London, London, UK; cThe Mater Misericordiae University Hospital, Dublin, Ireland; dWilliam Harvey Analysis institute, Queen Mary University of London, London, UKIntroduction: Urinary extracellular vesicles (uEVs) (exosomes, microvesicles and apoptotic bodies) have prospective as BTNL2 Proteins Source diagnostic and prognostic biomarkers. In atherosclerosis, the underlying lead to of heart attack and stroke, EV release may be dysregulated and their contents can mediate pro-inflammatory effects. A number of markers have been previously identified on uEV including exosome markers CD63 and CD9, CD45 (leukocyte marker), CD61 (platelet marker), CD14 (monocyte/macrophage marker) and / integrins. The selectively packaged cargo of these membrane bound carriers include microRNAs (miRs). miR-21 and miR-155 are key regulatory miRs which are upregulated in immune cells and are released in EVs following exposure to pro-inflammatory stimuli. miR-155 has been reported to possess pro-atherogenic effects and miR-155 deficiency in murine models results in reduced atherosclerotic lesion burden.ISEV2019 ABSTRACT BOOKMethods: Urine was collected from individuals diagnosed with coronary artery disease (CAD), Aminopeptidase N/CD13 Proteins Accession classified as symptomatic (non-ST-elevation myocardial infarction, STelevation myocardial infarction or unstable angina) or asymptomatic (stable angina). uEVs from symptomatic and asymptomatic patients had been isolated via benchtop centrifugation. The concentration and size of uEVs have been analysed through the NanoSight NS300 (n = 15 per group). The expression of miR-155 and miR-21 was investigated by RT-qPCR (n = 10 per group). uEV surface marker expression was analysed by ImageStreamX MK2 Imaging Flow Cytometer (12 per group). Results: uEV concentration in symptomatic individuals (median; 6.46E+9 particles/mL) was significantly decreased (p 0.05) in comparison to asymptomatic individuals (median; 1.25E+10 particles/mL). CD11B+ uEVs were elevated and CD16+ uEVs were decreased in the symptomatic individuals (p 0.01). Moreover, the concentration of CD45+ EVs have been improved in symptomatic sufferers (p 0.001). Though uEV miR-21 was unchanged, miR-155 expression was drastically elevated within the symptomatic group (p 0.05). Summary/Conclusion: uEV concentration, miR-155 expression and surface marker expression have diagnostic and prognostic potential. As CAD severity increases, uEV concentration is reduced, surface marker expression is altered and uEV miR-155 expression is increased. Funding: The Irish Study Council.OT01.Circulating extracellular vesicle-associated microRNAs as predictive biomarkers of cardiovascular complications in end-stage renal disease Dakota D. Gustafsona, Jessica Fitzpatrickb, Jason Fishc and Rulan Parekhba Division of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; bChild Wellness Evaluative Sciences, Investigation Institute, The Hospital for Sick Young children,.