Tients with diabetes. Solutions: Patients at Concord Hospital with suspected CAD gave written informed consent

Tients with diabetes. Solutions: Patients at Concord Hospital with suspected CAD gave written informed consent and had been administered RIPC (sphygmomanometer on the arm, 3 five min cycles, n = 31) or sham (n = 29) just before angiography, with recruitment ongoing. Blood was collected pre- and immediately post-RIPC/sham and plateletfree plasma generated. International coagulation/fibrinolytic prospective was measured by overall haemostatic possible assay (Reddel et al. Thromb Res. 2013; 131(five): 457462) and several fibrinolytic components by ELISA. EV wereUniversity College Dublin, Dublin, Ireland; bQueen Mary University of BTLA/CD272 Proteins Purity & Documentation London, London, UK; cThe Mater Misericordiae University Hospital, Dublin, Ireland; dWilliam Harvey Study institute, Queen Mary University of London, London, UKIntroduction: Urinary extracellular vesicles (uEVs) (exosomes, microvesicles and apoptotic bodies) have potential as diagnostic and prognostic biomarkers. In atherosclerosis, the underlying cause of heart attack and stroke, EV release is usually dysregulated and their contents can mediate pro-inflammatory effects. A number of markers have been previously identified on uEV which includes exosome markers CD63 and CD9, CD45 (leukocyte marker), CD61 (platelet marker), CD14 (monocyte/macrophage marker) and / integrins. The selectively packaged cargo of these membrane bound carriers incorporate microRNAs (miRs). miR-21 and miR-155 are crucial regulatory miRs which can be upregulated in immune cells and are released in EVs following exposure to pro-inflammatory stimuli. miR-155 has been reported to possess pro-atherogenic effects and miR-155 deficiency in murine models leads to reduced atherosclerotic lesion burden.ISEV2019 ABSTRACT BOOKMethods: Urine was collected from individuals diagnosed with coronary artery illness (CAD), classified as symptomatic (non-ST-elevation myocardial infarction, STelevation myocardial infarction or unstable angina) or asymptomatic (steady angina). uEVs from symptomatic and asymptomatic sufferers have been isolated by way of benchtop centrifugation. The concentration and size of uEVs have been analysed via the NanoSight NS300 (n = 15 per group). The expression of miR-155 and miR-21 was investigated by RT-qPCR (n = 10 per group). uEV surface marker expression was analysed by ImageStreamX MK2 Imaging Flow Cytometer (12 per group). Benefits: uEV concentration in symptomatic sufferers (median; six.46E+9 particles/mL) was significantly decreased (p 0.05) in comparison to asymptomatic patients (median; 1.25E+10 particles/mL). CD11B+ uEVs were elevated and CD16+ uEVs were decreased inside the symptomatic patients (p 0.01). Moreover, the concentration of CD45+ EVs have been enhanced in symptomatic patients (p 0.001). Even though uEV miR-21 was unchanged, miR-155 expression was considerably increased inside the symptomatic group (p 0.05). Summary/Conclusion: uEV concentration, miR-155 expression and surface marker expression have diagnostic and prognostic prospective. As CAD severity increases, uEV concentration is lowered, surface marker expression is altered and uEV miR-155 expression is improved. Syndecan-2/CD362 Proteins manufacturer Funding: The Irish Research Council.OT01.Circulating extracellular vesicle-associated microRNAs as predictive biomarkers of cardiovascular complications in end-stage renal disease Dakota D. Gustafsona, Jessica Fitzpatrickb, Jason Fishc and Rulan Parekhba Division of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; bChild Health Evaluative Sciences, Research Institute, The Hospital for Sick Youngsters,.