Location of 12,491.72 hectares inside the West Zone on the municipality of Rio de Janeiro

Location of 12,491.72 hectares inside the West Zone on the municipality of Rio de Janeiro [24]. Because of this, a number of initiatives were proposed, aiming to mitigate the effects of human occupation in this environment, like the implementation of a biological station named Esta o Biol ica FIOCRUZ Mata Atl tica (EFMA: Fiocruz Atlantic Forest Biological Station). The EFMA is really a a part of the campus FIOCRUZ Mata Atl tica (CFMA–FIOCRUZ Atlantic Forest Campus), and is at present an environmentally protected location surrounded by low-income communities [257]. Within this area, a number of scientific research projects have already been created, which includes the monitoring of fauna [26] and its parasites [17,28]. In EFMA, infections by trypanosomatids have been described in diverse hosts, for example bats, dogs, marsupials, and humans [17,25,27,29]. Remarkably, two new Trypanosoma species have been described within this area–T. janseni and Trypanosoma caninum, [17,29]–showing that this region, despite the fact that fairly Goralatide Purity little, may well nevertheless present unknown trypanosomatid diversity. In this study, we evaluated trypanosomatid infections in rodents and marsupials collected inPathogens 2021, ten,3 ofareas from EFMA with unique habitat characteristics in accordance with the amount of anthropic influence. Infections had been detected, employing parasitological, molecular, and serological assays, and parasites have been identified by DNA sequence evaluation. two. Final results 2.1. Smaller Mammals and Their Sampling Regions The species Didelphis aurita (Wied-Neuwied, 1826) widely prevailed inside the study area (n = 70), followed by Akodon cursor (Winge, 1887) (n = 7), Rattus rattus (Linnaeus, 1758) (n = 7), Marmosa paraguayana (Tate, 1931) (n = four), Oligoryzomys nigripes (Olfers, 1918) (n = two), Monodelphis americana (M ler, 1776) (n = 1), and Metachirus myosurus (Temminck, 1824) (n = 1). By far the most captured species, D. aurita, was collected in all expeditions: 19 in July 2012, 11 in November 2012, 9 in April 2013, 15 in July 2013, 15 in November 2013, and five in April 2014, including the four recaptures. A substantially larger number of smaller mammals captured was observed in peridomicile location A1 (n = 51) than in the other locations; namely, transition area A2 (n = 32) and preserved forest location A3 (n = 11) (two = 12.372, p = 1.2607E-05, df = two). two.two. Infection Prices of Trypanosomatids Regardless of the variations observed in the quantity of collected folks, we didn’t observe a considerable distinction in trypanosomatid prevalence amongst the different environments: A1 (36/50, 72 , self-confidence interval: 57.53.7), A2 (23/30, 76.7 , CI: 57.70.1), and A3 (11/9, 81.8 , CI: 48.27.7) (2 = 0.07819, p = 0.96166, df = two) (Table 1). Seventy-five specimens of marsupials and sixteen specimens of rodents collected had been analyzed for trypanosomatids, totaling ninety-one individuals. Thinking of all the host species, the total trypanosomatid prevalence was 74.7 (CI: 64.53.three). Trypanosomatid prevalence was similar for marsupials (76 , CI: 64.75.1) and rodents (68.7 , CI: 41.38.9), devoid of important distinction (two = 0.054569, p = 0.8153, df = 1). No significant JNJ-42253432 manufacturer difference was observed in trypanosomatid prevalence among male (73.6 , CI: 59.74.7) and female (76.3 , CI: 59.88.five) hosts (2 = 0.01261, p = 0.91059, df = 1).Table 1. Rodents and marsupials captured in 3 environments (peridomicile–A1, transition–A2, and preserved forest–A3) at EFMA, Rio de Janeiro (RJ), Brazil, between 2012 and 2014, and their infection prices by trypanosomatids. Order (n) Rodentia (16).