Ed Signal b Pulsed nESI Pulsed nESI Pulsed nESI Pulsed nESI Pulsed nESI nESI nESI Pulsed nESI nESI nESI in nESI nESI 0.ten Pulsed nESI nESI Ubq 9 Pulsed nESI9 nESI 0.11 Pulsed nESI 0.10 eight 2.83 0.11 1.10158 1580.05 8.99 0.13 9.19 0.05 eight.99 0.13 bq 9 eight 8 two.83 158 9.19 0.05 Ubq two.83 0.11 1.10 0.10 1.ten 9.19 eight.99 0.13 Ubq1313 9 1313 1.75 0.08 2.831.01 0.10 1.ten 0.10 13.2 74.0 81.75 three 9.19 0.05 Cyt C 13 1.75 0.08 1.0174.0 13.2 0.21 12.9 0.13 ytCyt C C 74.0 0.ten 0.21 0.08 0.110.10 1.01 13.2158 0.21 12.9 0.07 12.9 0.07 eight.99 0.07 CytMyo C 13 13 0.08 0.09 0.10 74.0 13.two 0.08 0.21 12.9 0.04 18 three.04 0.09 1.752.07 0.09 1.01 0.09 17.four 47.1 18 3.04 0.09 two.0747.1 17.40.04 17.3 0.07 Myo 1818 1818 47.1 0.08 Myo three.04 0.09 0.09 2.07 17.447.1 0.08 17.3 0.08 17.3 0.04 17.3 0.04 Myo 18 18 three.04 2.07 0.09 17.4 CAII 31 2.02 0.10 0.72 0.06 31.eight 1841.03 34.0 0.79 31.eight 1.03 34.0 AII 31 184 0.06 1.03 CAII 2.02 4 0.ten 0.100.06 0.72 84 0.72 31.8184 34.0 0.79 34.0 0.79 CAII 31 31 3434 2.02 0.ten 2.020.72 0.06 34 31.8 1.03 0.a a a Values BI-0115 Protocol correspond for the imply typical deviation of at deviation replicate three replicate b p-value 0.05.b Values correspond Valuesmean common deviation standardleast 3 of at leastmeasurements; measurements; p-value 0.05. toathe correspond to the mean of no less than 3 replicate measurements; b p-value 0.05. bTable units), percentage able two. Most abundant chargeabundant ion countthe theion count of your most abundant charge units), percentage raise in improve in in Table two. MostTable 2. 2. Most abundant charge state, most abundant charge state (arbitrary state (arbitraryincrease in abundant charge state, count of of mostcount of the most abundant charge state (arbitrary units), percentage raise Most state, ion charge state, ion abundant charge state (arbitrary units), percentage ion count, and average charge cytochrome C, myoglobin, andand carbonic anhydraseobtained from II obtained from state of ubiquitin, cytochrome C, myoglobin, and carbonic anhydrase n ion count, and average charge state ubiquitin, count, and typical charge state of of ubiquitin, cytochrome C, myoglobin, carbonic anhydrase II II obtained from ion count,and average charge state of ubiquitin, cytochrome C, myoglobin, and carbonic anhydrase IIproteins. from pulsed pulsed and present nESI mass current nESI a solution mixture containing all 4 proteins. Refer obtained ulsed andand traditional direct current nESI mass spectra of mass spectra ofcontainingmixture proteins. Referfourto Figure Refer to Figure pulsed standard directconventional directspectra of a resolution mixture a answer all 4 containing all to Figure andfor the corresponding mass spectra. 5 traditional direct current nESI mass spectra of a answer mixture containing all four proteins. Refer to Figure five for or the the corresponding mass spectra. five for corresponding mass spectra. the corresponding mass spectra.Values correspond to the mean common deviation of a minimum of three replicate measurements; p-value 0.05.three.four. Mechanistic three.4.3.four. Mechanistic Considerations Considerations Mechanistic Considerations The effects of electrophoretic mobility on Alvelestat Technical Information around the abundances ions formed in pulsedformed in pulsed The effects with the effects of electrophoreticabundances of of ions formed in pulsed electrophoretic mobility the mobility on the abundances of ions and direct was examined for examined for ions with unique mobilities. and direct present nESI was examined for ions with significa.
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