RFX5 Antibody (3B8) Summary
Immunogen |
RFX5 (NP_000440 516 a.a. – 616 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ERPGPMGEAEKGAVLAQGQGDGTVSKGGRGPGSQHTKEAEDKIPLVPSKVSVIKGSRSQKEAFPLAKGEVDTAPQGNKDLKEHVLQSSLSQEHKDPKATPP
|
Specificity |
RFX5 – regulatory factor X, 5 (influences HLA class II expression)
|
Isotype |
IgG2a Kappa
|
Clonality |
Monoclonal
|
Host |
Mouse
|
Gene |
RFX5
|
Purity |
IgG purified
|
Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
Dilutions |
|
Application Notes |
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
|
Packaging, Storage & Formulations
Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
Buffer |
PBS (pH 7.4)
|
Preservative |
No Preservative
|
Purity |
IgG purified
|
Notes
Quality control test: Antibody Reactive Against Recombinant Protein.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for RFX5 Antibody (3B8)
- DNA-binding protein RFX5
- Regulatory factor X 5
- regulatory factor X, 5 (influences HLA class II expression)
Background
A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.