Product Name :
Rabbit anti-Oct-4 Polyclonal Antibody
Synonym :
POU domain; class 5; transcription factor 1; Oct-3/4; Oct-4A; Oct4A; Oct 4A; Octamer-binding transcription factor 3; Octamer-4; Octamer-3; Oct-3; NF-A3; Otf-3; Otf3; MGC22487; Oct 3; Oct 4; OCT3; Oct4; Octamer binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding protein 3; Octamer-binding protein 4; OTF 3; OTF 4; OTF-3; OTF3; OTF4; PO5F1_HUMAN; POU class 5 homeobox 1; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU type homeodomain containing DNA binding protein; POU5F1
Host :
Rabbit
Species Reactivity:
Human, Mouse, Rat
Specificity :
Oct-3/Oct-4
Predicted Reactivity:
Dog, Pig, Cow
Applications :
IHC-P=1:100-500, Flow-Cyt=1ug/Test
Immunogen:
KLH conjugated synthetic peptide derived from human OCT-4:201-300/360
Concentration :
1mg/ml
Purification :
affinity purified by Protein A
Clonality:
Polyclonal Antibody
Storage Temp.:
Store at -20 ° C for one yearAvoid repeated freeze/that cycles
Research areas :
Cell biology Immunology Chromatin and nuclear signaling Stem cells Epigenetics
Background :
Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
UniProt :
P20263
Additional information:
Product Details FAQ Citations(1) Video Pictures Documents |Overview |Synonym POU domain; class 5; transcription factor 1; Oct-3/4; Oct-4A; Oct4A; Oct 4A; Octamer-binding transcription factor 3; Octamer-4; Octamer-3; Oct-3; NF-A3; Otf-3; Otf3; MGC22487; Oct 3; Oct 4; OCT3; Oct4; Octamer binding protein 3; Octamer binding protein 4; Octamer binding transcription factor 3; Octamer-binding protein 3; Octamer-binding protein 4; OTF 3; OTF 4; OTF-3; OTF3; OTF4; PO5F1_HUMAN; POU class 5 homeobox 1; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU type homeodomain containing DNA binding protein; POU5F1 |Host Rabbit |Specificity Oct-3/Oct-4 |Species Reactivity Human, Mouse, Rat |Predicted Reactivity Dog, Pig, Cow |Applications IHC-P=1:100-500, Flow-Cyt=1ug/Test |Immunogen KLH conjugated synthetic peptide derived from human OCT-4:201-300/360 |Properties |Concentration 1mg/ml |Purification affinity purified by Protein A |Clonality Polyclonal Antibody |Isotype IgG |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |Research areas Cell biology Immunology Chromatin and nuclear signaling Stem cells Epigenetics |Target |Background Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state. |Cellular localization Cell nucleus; |UniProt P20263 | Sample: Embryo (Mouse) Lysate at 40 ugTestis Cell Lysate at 40 ugNIH/3T3 (Mouse) Lysate at 40 ugHela(Mouse) Lysate at 40 ugRsc96 Cell(Rat) Lysate at 40 ug293T Cell(Human)Lysate at 40 ugSP2/0 Cell(Mouse) Lysate at 40 ugHL60 Cell(Human)Lysate at 40 ugPrimary: Anti- Oct-3/Oct-4 (abs120769) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30,39 kDObserved band size: 30,39kD| Sample: Testis Cell Lysate at 40 ugPrimary: Anti- Oct-3/Oct-4 (abs120769) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 30,39 kDObserved band size: 30,39kD| Sample: Embryo (Mouse)Lysate at 40 ugTestis (Mouse) Lysate at 40 ugNIH/3T3 (Mouse) Cell Lysate at 40 ugHela(Human) Cell Lysate at 40 ugRsc96(Rat) Cell Lysate at 40 ug293T(Human) Cell Lysate at 40 ugSP2/0(Mouse) Cell Lysate at 40 ugHL-60(Human) Cell Lysate at 40 ugPrimary: Anti-Oct-3/Oct-4 (abs120769) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 39 kDObserved band size: 39 kD |Tips:This product is for research use only. Not for use in diagnostic prodcedures.
Rabbit anti-Oct-4 Polyclonal Antibody
Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.Related websites: https://www.medchemexpress.com/antibodies.html
152121-30-7 site 1092939-17-7 manufacturer PMID:29939605 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
